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Image Search Results
Journal: bioRxiv
Article Title: The extrafollicular B cell response is a hallmark of childhood idiopathic nephrotic syndrome
doi: 10.1101/2023.09.11.557079
Figure Lengend Snippet: a. Minimal spanning trees (MST) of HC ( left ) and INS ( right ) B cells generated by FlowSOM clustering. The 14 metaclusters (M0-M13) are labelled on both MSTs. Arrowheads indicate key cell clusters expanded in INS b. Heatmap showing the geometric mean fluorescence intensities (gMFI) of the markers used for clustering in each metacluster. c. Proportions of each metacluster in HC and INS PBMC. d., e. Histograms of CD21, CXCR5, and Ki-67 in the six metaclusters enriched in INS ( d ) with quantification of the proportions of CD21 low CXCR5 + , and Ki-67 + cells in each metacluster ( e ); in d and e , p values were determined by Mann-Whitney tests (* p <0.05, ** p <0.01, *** p <0.001, **** p <0.0001). Each data point corresponds to a single donor. The yellow data point represents the child with glucocorticoid-resistant membranous nephropathy.
Article Snippet: Intracellular proteins were stained using the following antibodies: anti-human T-bet PE (1:20, 4B10, BioLegend), and
Techniques: Generated, Fluorescence, MANN-WHITNEY